The purpose of the present study was to examine if the length of yoga training may influence the use of cognitive reappraisal and expressive suppression (as emotion regulation strategies) and whether this relationship may be moderated by personality traits. Based on previous studies, we hypothesized that the link between the length of yoga practice and emotion regulation can rely most heavily on participants’ conscientiousness and extraversion levels. Ninety women in two groups participated in the study: those who have been practicing yoga for over a year and those who have been practicing for a shorter period of time. An Emotion Regulation Questionnaire was applied to measure the use the strategies of cognitive reappraisal and expressive suppression. Moreover, personality traits, based on the Big Five model were assessed. The results of the study provided support for our prediction: participants who engaged in yoga practice for a longer period of time (as compared to participants who practiced yoga for a shorter duration), reported using cognitive reappraisal more often. Furthermore, longer yoga practice was more beneficial than shorter practice especially for individuals with low levels of conscientiousness and extraversion. Thus, extraversion and conscientiousness seem to facilitate the process of drawing benefits from practicing yoga.